During the course of MDCK cyst maturation, CLIC4 was first found on the cell periphery, with gp135+/Rab11a+ apical vesicles (Fig. 4b,c), and then concentrated near the gp135-enriched AMIS juxtaposing Rab11a-labelled REs (Fig. 4d-g). Here, CLIC4 is linked to cyst.