Interestingly, the only cell line in which we could detect a cellular response to OCN variants was the rat insulinoma INS-1(832) cell line, in which bovine, mouse and human OCN displayed significant, concentration-dependent activity in the label-free cellular impedance assay as applied to the FlpIn-TREx-HEK293-mGPRC6A cells (Fig 7). Here, BGLAP is linked to pancreatic insulinoma.