Although there are no concentration determinations for Hsf1 in different tissues available to our knowledge, using the relative quantification data determined by mass spectrometry for 11 different cancer cell lines (Geiger et al., 2012), and assuming that these cells have a total protein concentration of about 150 mg/ml as determined for HEK293 cells (Gillen and Forbush, 1999), results in Hsf1 concentrations between 10 and 130 nM. Here, HSF1 is linked to cancer.