In case of gene–clinicopathological characteristic relationship, significant negative correlation was observed between SIRT3 versus N stage (r = -0.226**, p<0.01), SIRT4 versus N stage (r = -0.261***, p<0.001), MTUS1 versus N stage (r = -0.214*, p<0.05) and OGG1-2a versus N stage (-r = 0.378***, p<0.001) in HNSCC cases. The gene discussed is SIRT4; the disease is head and neck squamous cell carcinoma.