Analysis of GLI1 protein expression in SUFU-positive and SUFU-deficient human medulloblastoma cells (Figure 4E, 4F) and Sufu knockout mouse embryonic fibroblasts (Figure 4G) further corroborated the potent activity of DYRKi to inhibit GLI activity in SMO-inhibitor sensitive and resistant cells. The gene discussed is SMO; the disease is medulloblastoma.