Immunolocalization using a SPARC antibody demonstrated that 3 of the 4 TIC tumor transplants (As#3, As#6, Cd#4) generated from the non-SPARC transfected urospheres (TIC) had focal expression of the SPARC protein representing between 10 to 20% of the total tumor mass (Fig 4E and 4F, focal staining illustrated for As#3 and Cd#4). This evidence concerns the gene SPARC and neoplasm.