An identical immunolocalization protocol on TIC tumor transplants generated from the SPARC-transfected urospheres (TIC) showed focal expression of the SPARC protein in all 4 TIC tumor transplants with SPARC staining representing between 5 to 20% of the total tumor mass (Fig 4G and 4H, focal staining illustrated for As#3-SPARC and Cd#4-SPARC). This evidence concerns the gene SPARC and neoplasm.