Further, to investigate the role of mGluR network CNVs in the development of ASD in vulnerable syndromes, we studied children with 22q11.2 Deletion Syndrome, who all have deletion of the mGluR gene RANBP1. We identified children with 22q11.2DS (n = 75), including those with ASD (n = 25) and without ASD (n = 50), and looked for the rate of “second hits” in the mGluR network to determine if additional disease risk is incurred for ASD with double “hits” to the mGluR network. Here, RANBP1 is linked to 22q11.2 deletion syndrome.