To investigate whether olanzapine co-treatment can reverse the PCP-induced down-regulation ofn Akt-GSK signaling in vitro, we examined the mRNA expressions of GSK3β and Akt, and the protein expressions of Akt, p-Akt, GSK3β, and p-GSK3β in primary PFC cultures under PCP treatment or Olanzapine-PCP co-treatment. The gene discussed is AKT1; the disease is pneumocystosis.