To further confirm the presence of KSHV in endosome containing gradient fractions, fractions collected at different early time points (1, 5, or 30 min) during the course of infection were resolved by SDS-PAGE and subsequently Western blotted with antibodies directed against minor capsid protein KSHV ORF62-encoded triplex component I (TRI-1) for detecting the presence of viral capsids (Fig. 3). This evidence concerns the gene NR4A3 and infection.