Considering HDACi mediated up-regulation of NIS gene can have broader scope in thyroid and other cancer types, the possibility was tested by using several additional human cancer cell lines such as NPA and ARO (thyroid cancer), A2780 (ovarian cancer) and HT1080 (fibrosarcoma) over-expressing the pNIS-Fluc2.TurboFP plasmid. The gene discussed is SLC5A5; the disease is thyroid cancer.