EMC6 and osteosarcoma: Our previous report demonstrated that EMC6-mediated autophagy is associated with the activity of class III PI3K in the osteosarcoma U2OS cell line.23 Here, we also found that the treatment of 3-methyladenine (3-MA, an inhibitor of class III PI3K) could attenuate the LC3B-II accumulation in U87 cells induced by EMC6 overexpression (Supplementary Figures 5e and f).