Our data are in accordance with the recent report by Rucksaken [10] which demonstrated that CC sera contain AAbs reacting with enolase and heterogeneous nuclear ribonucleoprotein L. However, we were not able to detect AAbs to ribonuclease/angiotensin inhibitor 1 or to heat shock protein 70, as reported by the same authors. This evidence concerns the gene HNRNPL and cholangiocarcinoma.