On the one hand, CMTM3 expression, which was shown to be down-regulated in the ultracentrifugation uEVs (but not detected with Norgen extraction method), showed a significant reduction in PCa compared with BPH tissues, but this result was not reproduced in publicly available PCa datasets and exhibited only a modest trend in PCa cell lines (Supplementary Figure S3A–S3C). This evidence concerns the gene CMTM3 and posterior cortical atrophy.