TLR4 and malignant colon neoplasm: Immunohistochemistry analysis revealed that NF-κB p65 expression in colon cancer tissue increased significantly compared to normal colon tissue (Fig 3).Our results suggest a critical role of NF-κB as a signaling molecule in inflammatory process, which facilitates the expression and secretion of pro-inflammatory cytokines, leading to a series of inflammatory responses by TLR4 activation.