To confirm our hypothesis that, the highly level of TLR4 in colon cancer patients leads to induction of an inflammatory response mediated by multiple pathways specifically NF-κB and induce an acute secretion of inflammatory cytokines as such as IL-6, a comparative study of NF-κB expression was performed by immunohistochemistry on cancer colon tissues and normal matching tissues. Here, TLR4 is linked to malignant colon neoplasm.