In order to examine the impact of Tie2‐IFNα on established liver metastases, CB6 mice were injected intrahepatically (beneath the Glisson's capsule) with 5 × 103 CT26 CRC cells and transplanted 8 days later with Tie2‐GFP‐ or Tie2‐IFNα‐transduced HSPCs (see a schematic representation of the experimental strategy in Fig 5A). This evidence concerns the gene IFNA1 and colorectal carcinoma.