Particularly, CaCo2 cells show a TP53 point mutation determining undetectable p53 protein by immunohistochemistry, HCT116 cells do not show TP53 mutation, HT29 and SW480 cells show TP53 point mutation, p53R273H and p53R273H/P309S respectively, which induces the mutated proteins to attain ‘gain of function’ (GOF), dynamically involved in cancer development and progression [39]. This evidence concerns the gene TP53 and cancer.