LEO1 and cancer: However, these findings diverge to some extent from observations in fission yeast, where depletion of multiple subunits led to significant decreases in H3K4me2/me3, and in other cancer cell lines, in which Leo1 silencing reduced H2Bub and Ski8 and Ctr9 depletion diminished H3K4me1/me3 and H3K79me2 [2, 4, 16].