ROCK1 and infection: Infection of cells with adenovirus is not 100% efficient, and when the growth of Rock1∆/∆;Rock2∆/∆ cells was monitored over a longer period of time, these cells eventually recovered their ability to proliferate (Figure 1—figure supplement 1B), but western blot analysis revealed that these cells express ROCK1 and 2 in equivalent levels to wild type cells (data not shown) and thus likely originated from uninfected cells.