Rhabdomyosarcoma (RMS) and Glioma were induced by co-injecting plasmids of UAS-mCherry-KrasG12V and Rag2-Gal4VP16 or GFAP-Gal4VP16 to Tg(fli1a:eGFP):p53 +/− transgenic zebrafish embryos at the one cell stage as previously described24 (Supplementary Fig. S4). Here, GFAP is linked to rhabdomyosarcoma.