In the present study, we examined the expression of notch signaling receptor NOTCH-1, NOTCH-3, NOTCH-4, ligands JAG1 and DLL-4 along with the downstream targets Hes-1 and Hey-1 to determine the efficacy of a combined therapeutic strategy with endostatin/cyclophosphamide (CTX) to normalize the tumor micrangium associated with colon cancer. Here, NOTCH4 is linked to malignant colon neoplasm.