To investigate the biological function of ARHGDIA in gliomas, we constructed a recombinant adenovirus encoding a green fluorescent (GFP) fusion protein—GFP-ARHGDIA protein (AD-ARHGDIA, 55 kDa)—and used the recombinant adenovirus encoding GFP protein (AD-GFP, 28 kDa) as a negative control. This evidence concerns the gene ARHGDIA and central nervous system cancer.