Western blotting analysis and transwell assays demonstrated that both the protein level of ARHGDIA and the number of migrating cells after the co-transfection of ARHGDIA siRNA and miR-151-5p/miR-16 inhibitors (anti-miR-151-5p/anti-miR-16) were closer to that of the control level than after individual transfection of miR-151-5p/miR-16 inhibitors Knockdown of ARHGDIA rescued the inhibitory effects of miR-151-5p/miR-16 inhibitors on glioma cell migration. The gene discussed is ARHGDIA; the disease is central nervous system cancer.