In conclusion, the results from this study demonstrate that honokiol inhibits the migration capacity of melanoma cells and that this process involves: (i) the inhibitory effect of honokiol on oxidative burst/oxidative stress in melanoma cells, (ii) the suppression of NADPH oxidase activity by blocking interaction between cytosolic and membrane-bound proteins responsible for ROS generation, and that these data were supported by the bioluminescence imaging of internal body organs to detect luciferase-tagged melanoma cells. This evidence concerns the gene FMO5 and melanoma.