Based on different APC genotypes, this study aims to investigate the function and regulatory mechanism of TLRs signal pathway in colon cancer growth, prove the connection of TLRs/NF-κB pathway activation and APC/GSK-3β/β-catenin, elucidate the influence of TLRs/NF-κB activation on tumor, and provide a new thread for colon cancer prevention. Here, GSK3B is linked to colonic neoplasm.