To verify the proliferation changes of colon cancer cells with different APC genotypes after the LPS stimulation, LPS with different fold dilution series (0, 0.5, 1.0, 2.0, 5.0, and 10.0 μg/ml) were added to the HCT116, RKO, SW480, DLD-1 and HT29 cultures. The gene discussed is APC; the disease is malignant colon neoplasm.