In order to investigate the relationship between APC phenotypes and TLR4-mediated immune response in the development of colon cancer, we established stable APC knockdown cell lines, and to explore different biological behavior in different APC state under TLRs/NF-κB pathway activation, and to confirm that TLRs/NF -κB activation influences the development and progression of colorectal cancer in APC mutant population. The gene discussed is NFKB1; the disease is colorectal cancer.