The result showed the infarction volume examined on T2-weighted images at 2 d after MCAO was significantly reduced in the 3 pre-treatment groups (E2: 109.54 ± 43.29 mm3, CD34+: 87.14 ± 40.20 mm3, E2+CD34+: 43.34 ± 20.04 mm3) when compared with no treatment group (174.15 ± 32.55 mm3, p = 0.026, 0.003, and < 0.001, respectively) (Fig 2). The gene discussed is CD34; the disease is infarction.