RB1 and hepatocellular carcinoma: To clarify the molecular mechanism by which JMJD5 regulates the G1-S transition in HCC cells, we used real-time RT-PCR to analyze the mRNA levels of known molecules involved in the G1-S transition, including RB, CDKN1A, CDK2 and CCNE, in MHCC-97H and L02 cells that were silenced for JMJD5.