Next, we immunostained the brain tumor sections with antibodies against gamma-tubulin, a marker of ciliary basal bodies, and SMO, a key component of the ciliary SHH signaling pathway that is transported into the cilium when the cilium responds to SHH, to determine whether there was any evidence that the cilia of distally located L0 cells had been responding to SHH prior to brain fixation. This evidence concerns the gene SHH and brain neoplasm.