Notably, as mentioned in introduction section, MEKK1 also played an important role in the regulation of NF-κB signaling, and it is worthy to examine whether NF-κB signaling was altered in our experimental settings, because of the central role of NF-κB signaling in facilitating the metastasis and growth of breast cancer (Huber et al. 2004; Biswas et al. 2004). The gene discussed is MAP3K1; the disease is breast cancer.