Lgr5 overexpression appeared to be significantly associated with deep invasion of CRC (OR = 0.39, 95 % CI: 0.17–0.87; P = 0.002), lymph node metastasis (OR = 0.45, 95 % CI: 0.26–0.76; P = 0.003), distant metastasis (OR = 0.37, 95 % CI: 0.22–0.62; P = 0.000), and AJCC stage (OR = 0.35, 95 % CI: 0.15–0.78; P = 0.01). This evidence concerns the gene LGR5 and metastatic malignant neoplasm in the lymph nodes.