AFP and liver cancer: In this investigation, human liver cancer cells HLE and Bel 7402 cells were selected for transfection with AFP overexpression vectors (pcDNA3.1‐afp) and silenced vectors (AFP‐siRNA), respectively; both scratch assay and transwell chamber assay indicated that overexpression of AFP in HLE cells was able to significantly repair the scratch area and penetrate the transwell chamber pores in contrast with control cells; whereas the capacity of repairing, migration and invasion of Bel 7402 cells was significantly declined while transfected with AFP‐siRNA vectors.