The aims of the current study were: 1) to characterize the IGF and mTOR pathways, 2) to evaluate the effect of IGF1R/IR blocking, using the dual inhibitor OSI-906, and mTOR blocking, using several mTORi as single agents and 3) to evaluate wheter the combination of the two categories of compounds has an additive effect in the regulation of cell secretion, proliferation, migration, invasion and cell cycle in two HCC cell lines. Here, INSR is linked to hepatocellular carcinoma.