In the attempt to define the role of mTOR and IGF pathways in HCC, RT-qPCR was performed to quantify the messenger expression level of mTOR, 4eBP1, p70S6K, IGF1, IGF2, IGF1R, IGF2R and IR (isoforms A and B), in THLE-2, HepG2 and HuH-7 cell lines, in normal liver and in HCC and peritumoral tissues. This evidence concerns the gene RPS6KB1 and hepatocellular carcinoma.