In order to further assess the functionality of IL-17R in GSCs and to elucidate the underlying mechanisms, IL-17R+ GSCs were isolated from primary gliomas via flow sorting and seeded at 0.25 × 106 cells/ml/well into a 12-well plate in neurosphere medium, and then cultured with IL-17 (100 ng/ml) in the presence of IL-17R/IL-6R blocking antibodies (1 μg/ml) and specific signaling inhibitors, as indicated. Here, IL17RA is linked to glioma.