To eliminate the possibility that a mechanism independent of the aqueduct induces non-communicating hydrocephalus, we deleted Yap in other parts of the neuroepithelium by Emx1 Cre (expressed only in dorsal cortex) and hGFAP Cre (radial glia progenitors but not in the ventral midbrain). Here, EMX1 is linked to Hydrocephalus.