Although homologues of Lgl1 and aPKC in Drosophila regulate Hippo (hpo) kinase activity by modulating dRassf inhibition of hpo39, we found that double conditional knockout of Mst1/2, the mammalian homolog of hpo, with Nestin Cre did not elicit a hydrocephalus phenotype (unpublished data). Here, NES is linked to Hydrocephalus.