To confirm that JunB knockout reduced the incidence of lung metastasis in the animal model, the in vivo study was repeated to measure the weight of each lung and the area occupied by metastatic HNSCC cells in the lung at 78 days after cell inoculation (6 mice were used for the control group and 8 mice for the JunB KO group). This evidence concerns the gene JUNB and head and neck squamous cell carcinoma.