On the other hand, because cholinergic neurons in cerebral cortex belong to projection neurons which the basal forebrain cholinergic neuron complex such as the nucleus basalis of Meynert (NMB) [49,50], the medial septal nucleus and the diagonal band nuclei projects to, and the function of cerebral cortex is associated with a cognitive, learning and memory functions, VAChT in cerebral cortex will be suitable to the target for early diagnosis of Alzheimer’s disease. This evidence concerns the gene SLC18A3 and early-onset autosomal dominant Alzheimer disease.