PRKN and movement disorder: We also noticed that aged mice showed the characteristic phenotype of movement disorder (Fig. S4), which younger mice have less obvious phenotypes, suggesting the aged related cellular events, such as protein oxidation and aggregation, or altered dopamine metabolisms may be involved (Goldberg et al., 2003), and our data directly showed that parkin mediated the aggregated p62 degradation in cells (Fig. S5).