Therefore, to investigate possible tumor-derived soluble factors induced by irradiation that contributed to the decrease of IL-12 in DCs, DCs were isolated from mouse bone marrow cells (BMDCs) that were differentiated in media containing IL-4 (10 ng/mL) and GM-CSF (10 ng/mL) for 6 days and then, these were incubated for 72 h in supernatants of MIH-2 cell irradiated with or without 10 Gy. This evidence concerns the gene CSF2 and neoplasm.