In addition, EGF stimulation resulted in simultaneous TOPK and c-Jun phosphorylation, both in HEK293 cells and H358 cells that expressed ectopic TOPK (Figure 5C) and in EGFR-TKI-resistant A549 and H1975 lung cancer cells that expressed high endogenous TOPK (Figure 5D). This evidence concerns the gene PBK and lung carcinoma.