At 48 h after transfection of Artificial hTERT promoter-Bax-Anti Bcl2 combination module or negative control, the relative activity of caspase-3 and the apoptosis ratio in bladder cancer 5637 cells, T24 cells, and NHF were detected by the caspase-3 enzyme-linked immunosorbent assay (ELISA) assay and the flow cytometry analysis. This evidence concerns the gene CASP3 and urinary bladder carcinoma.