To confirm whether ETS-1 is associated with the driven efficiency of artificial hTERT promoter, bladder cancer cells, 5637, T24, UMUC-3 and RT4, were cultivated and co-transfected with the ETS-1 shRNA and artificial hTERT promoter reporter module or the negative control shRNA and artificial hTERT promoter reporter module. The gene discussed is ETS1; the disease is urinary bladder cancer.