Compared with the group which was co-transfected with negative control shRNA, the activity of artificial hTERT promoter was respectively decreased by nearly 69 % in bladder cancer 5637 cells, 77 % in bladder cancer T24 cells, 32 % in bladder cancer UMUC-3 cells and 80 % in bladder cancer RT4 cells in the group which was co-transfected with ETS-1 shRNA (Fig. 3). The gene discussed is ETS1; the disease is urinary bladder cancer.