As shown in Fig. 4C,D, expression of nuclear STAT3 and nuclear p-STAT3 (S727) but not cytoplasmic STAT3 and cytoplasmic pSTAT3 (S727) of tumor tissues from CCA patients with DM were significantly higher than those with non-DM (P < 0.05). This evidence concerns the gene STAT3 and cholangiocarcinoma.