Proteomic analysis of AKT, ERK1/2, MEK1/2, EGFR and c-MET phosphorylation was conducted for normal and tumor ischemic tissue samples from all 20 patients, and relative quantification of phosphorylation was calculated by determining the AUC of annotated peaks in relation to each other (% area). The gene discussed is EGFR; the disease is neoplasm.