NCAPH2 and Alzheimer disease: In order to verify CpG methylation levels in the NCAPH2/LMF2 gene region, we conducted pyrosequencing for an AD group, aMCI group and a NC group having 30, 28 and 30 age- and sex-matched subjects, respectively, which included the subjects whose samples were analyzed in the Illumina Infinium HD Methylation Assay (Fig 1).