The overexpression of COL3A1 protein in CRC, based on the positivity by IHC and pixel analysis, was significantly associated with grade (χ2 = 6.731, p = 0.029), overall stage (χ2 = 8.438, p = 0.004) and T stage (χ2 = 6.923, p = 0.012) (Table 2). This evidence concerns the gene COL3A1 and colorectal carcinoma.