The present study investigated differentially expressed genes in JGM-treated adult BPH females based on the gene expression profiles, and identified adipose triglyceride lipase gene (Atgl) as the target for gene silencing by RNAi because it was highly expressed in JGM-treated females and because it plays critical roles in energy balance leading to reproduction. Here, PNPLA2 is linked to benign prostatic hyperplasia.