Immunofluorescence staining of Ki67 and cleaved caspase 3 of organoids derived from fresh and DMSO frozen BT474 tumors treated for 72 hr with anti-cancer drugs, revealed increased cleaved caspase 3 expression in organoids treated with paclitaxel, trastuzumab, XL147, and H + P + X (Fig. 3f). This evidence concerns the gene CASP3 and cancer.