SOD1 and amyotrophic lateral sclerosis: As a consequence, even minor changes of solution conditions such as pH will trigger significant shifts of HSQC peaks due to the changes of the chemical environments, although the conformation may remain largely unchanged [58], as we previously observed on the disordered ALS-causing SOD1 mutant [38], P56S-MSP domain [62] and isolated dengue NS3 protease domain [63].