We observed a significant reduction of Runx1 transcriptional activity in DN/Runx1 transfected tumor cells (Figure 2A and 2B), which resulted in a significant downregulation of Rspo3 expression and secretion (Figure 2C–2E: LM3 cell line and 2F: MDA-MB-231 cell line). The gene discussed is RSPO3; the disease is neoplasm.