RUNX1 and breast cancer: In addition, to determine whether induction of Foxp3 expression may result in inhibition of Runx1 target genes expression in mammary tumor cells (LM3 cells), which show low levels of Foxp3 (Supplementary Figure 1), were treated with low doses of anisomycin which is able to upregulate Foxp3 expression in mammary epithelial cell lines (Figure 4G) by c-Jun and ATF-2 activation [34].