We found that the expression level of HIF-1α, detected by realtime-PCR and western blot, increased in RPS7-silenced CRC cells but decreased in RPS7-overexpressing CRC cells, compared with the corresponding control cells, suggesting that RPS7 negatively regulated the expression of HIF-1α in CRC cells (Figure 3B and 3C). The gene discussed is HIF1A; the disease is colorectal carcinoma.