Measurements of the virus titer in the supernatants of infected cells indicated that significant inhibition by the overexpression of RyDEN was observed in HCV, WNVKUN, and CHIKV, but not in poliovirus, EV71, or HIV-1 infections, as compared to that in control protein-expressing cells (Fig 10A). The gene discussed is SHFL; the disease is HIV-1 infection.