By triple immunostaining, we show in control tissue, sparse overlapping of RAGE-S100B, moderate RAGE-CML and RAGE-HMGB1 immunostaining (Figures 4A,B and 5A,C) whereas in ALS tissue, we found a high degree of overlapping expression patterns for all ligands with RAGE (Figures 4A,B and 5B,D). This evidence concerns the gene HMGB1 and amyotrophic lateral sclerosis.