Doxorubicin (DOX) and Cy5.5-decorated AuNP are integrated into matrix metalloproteinase-2 (MMP-2) degradable gelatin nanoparticles. DOX and Cy5.5 linked to AuNPs through a hydrazine bond to enable pH-triggered cargo release. Active glioma targeting is enabled using surface modification with RRGD, a tandem peptide. At glioma sites, MMP-2 degrades the gelatin nanoparticles and the release of DOX and Cy5.5 is triggered by low pH. This evidence concerns the gene MMP2 and central nervous system cancer.